Influence of Trichoderma asperellum and Bacillus subtilis as biocontrol and plant growth promoting agents on soil microbiota

Abstract Purpose The manipulation of soil microbiota can involve changes in microbial diversity and activities, it is carried out for practical purposes. be modified by the inoculation beneficial microorganisms into or agricultural management practices. This study provides information on influence introducing indigenous microbiota. aim this was to determine how biocontrol plant growth promoting agents Trichoderma asperellum Bacillus subtilis their consortium affect without placing emphasis as a determinant change. Methods Experimental samples were treated with B. T. consortium. shift number bacterial fungal CFUs determined. Biolog EcoPlate assay demonstrated metabolic activity soil. concentration genus, Firmicutes, Gammaproteobacteria Acidobacteria DNA determined molecular methods. A correlation analysis performed between microbiological data. Results Soil treatment , pathogenic fungus Botrytis cinerea changed amplified fragments certain taxa. showed that added did not significantly community Shannon-Wiener biodiversity index but change utilization carbohydrates, complex carbon compounds organic phosphorus compounds. Conclusion Introduced survive during 60-day experiment composition functionality populations. Whereas are inhibitors could further positively specific crops.